Department of Regenerative Medicine and Cell biology

Ric Boockfor

Fredric R. Boockfor, Ph.D.
Associate Professor



Room 645, Basic Science Building
Office: (843) 876-3526
Lab: (843) 876-3546


Email: boockfor@musc.edu

Education:

BS Pennsylvania State Univ. 1973
MS Duquesne University 1976
PhD Clemson University, 1982
Postdoctoral Fellow at Michigan St. Univ and Iowa St. Univ.

Research Interest:

My primary research interest is the study of the specific cellular and molecular processes underlying the control of endocrine cell secretion. Two important cell models, prolactin secreting cells of the anterior pituitary and gonadotropic hormone releasing hormone (GnRH)-secreting neurons of the hypothalamus are used in investigations in the laboratory. Our use of these cells in conjunction with a recently developed promoter driven luciferase detection system enables us to study, in real-time, prolactin or GnRH gene expression at the level of the single cell. Our findings with this approach, when coupled with other single cell fluorescent techniques such as measurement of exocytotic release or measurement of changes in intracellular calcium have provided a great deal of insight on the interplay of nuclear and cytoplasmic processes that are critical to the control of hormone secretion. Information from our studies is not only important for understanding of multiple facets of endocrine function in individual cells, but also provides an awareness of various aspects of gene expression that are fundamental to the control of all cells, endocrine and non-endocrine alike.

Recent Publications:

  1. Bose S and Boockfor F. R. Episodes of Prolactin Gene Expression in GH3 Cells Are Dependent on Selective Promoter Binding of Multiple Circadian Elements.
    Endocrinology 151;2287-2296, 2011.
  2. Bose S, R. Vazquez-Martinez and F. R. Boockfor. Administration of connexin-43 siRNA abolishes secretory pulse synchronization in GnRH clonal cell populations. Molecular and Cellular Endocrinology 314:75-83, 2010
  3. Bullesbach EE, F. R. Boockfor, G Fullbright, C. Schwabe. Cryptorchidism induced in normal rats by the relaxin-like factor inhibitor. Reproduction. 135:351-355, 2008.
  4. Leclerc G.M., S. Bose, and F. R. Boockfor. Specific GATA binding elements in the GnRH promoter are required for gene expression pulse activity: Role of GATA-4 ands GATA-5 in this intermittent process. Neuroendocrinology. 88:1-16, 2008.
  5. Leclerc, G. M. and F. R. Boockfor. Calcium influx and DREAM protein are required for GnRH gene expression pulsatility. Molecular and Cellular Endocrinology. 267:70-79, 2007.
  6. Leclerc, G. M. and F. R. Boockfor. Identification of a novel OCT1 binding site that is necessary for the elaboration of pulses of rat GnRH promoter activity. Molecular and Cellular Endocrinology. 245:86-92, 2006.
  7. Leclerc GM, Boockfor FR. Identification of a novel OCT1 binding site that is necessary for the elaboration of pulses of rat GnRH promoter activity. Mol Cell Endocrinol.;245:86-92., 2005.
  8. Leclerc GM, Boockfor FR. Pulses of prolactin promoter activity depend on a noncanonical E-box that can bind the circadian proteins CLOCK and BMAL1. Endocrinology.146:2782-90., 2005.
  9. Boockfor FR, Fidan M. Reverse hemolytic plaque assays: versatility in the study of secretion. Methods. 33:273-80, 2004
  10. Morris Buus R, Boockfor FR. Transferrin expression by placental trophoblastic cells. Placenta. 25:45-52, 2004
  11. Blake CA, Boockfor FR, Nair-Menon JU, Millette CF, Raychoudhury SS, McCoy GL. Effects of 4-tert-octylphenol given in drinking water for 4 months on the male reproductive system of Fischer 344 rats. Reprod Toxicol;18:43-51, 2004

 

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