Christine B. Kern, Ph.D.
Room 618, Basic Science Building
Office: (843) 792-9618
Lab: (843) 792-4279
BA Biology Hope College, Holland MI 1985
PhD Microbiology and Immunology University of Kentucky 1992
Postdoctoral Fellow at University of Cincinnati (J. Lingrel)
I am interested in the dynamic role of the extracellular matrix (ECM) during cardiovascular development and disease. One of my current research interests centers around our recent findings that have revealed that the ADAMTS (A Disintegrin-like And Metalloprotease domain with ThromboSpondin-type 1 motifs) proteases are critical for ECM remodeling during maturation of the semilunar valves and arterial wall of the aortic and pulmonary arteries. We are currently investigating changes in cell behavior and signaling that contribute to abnormal valve development and anomalies of disease in mouse models of ADAMTS deficiency. Since we are interested in elucidating the biomechanical circuitry involved in development and disease we focus on the use of mouse models and confocal imaging techniques to test our hypothesis in vivo and use supportive ex-vivo approaches to further investigate mechanistic pathways.
One of the most rewarding aspects of my scientific career is providing opportunities in my laboratory and in the classroom for students to explore their scientific interest and potential. I mentor undergraduates in the MUSC Student Undergraduate Research Program (SURP) and College of Charleston Students in the Honor’s College in their Bachelor’s Essays projects. I am also interested in educating and mentoring middle school and high school students in the public schools to encourage more career interest in the Science Technology Engineering and Math (STEM) careers. If you are interested in a research opportunity in my laboratory or collaborative educational interests please email.
American Heart Association- Scientist Development Grant: 2010-2013
ADAMTS9 in cardiac development and disease
NCRR COBRE pilot project:
Prrx1 a key regulator of the extracellular matrix during secondary palate development
HDAC regulation of the Ncx exchanger
Selected Recent Publications:
- Dupuis, L. E., H. Osinska, M. B. Weinstein, R. B. Hinton and C. B. Kern. "Insufficient Versican Cleavage and Smad2 Phosphorylation Results in Bicuspid Aortic and Pulmonary Valves." J Mol Cell Cardiol 60, (2013): 50-9.
- M. A. Cooley, V. M. Fresco, M. E. Dorlon, W. O. Twal, N. V. Lee, J. L. Barth, C. B. Kern, M. L. Iruela-Arispe, and W. S. Argraves. Fibulin-1 is Required During CardiacVentricular Morphogenesis for Versican Cleavage, Suppression of ErbB2 and Erk1/2 Activation, and to Attenuate Trabecular Cardiomyocyte Proliferation, Developmental Dynamics,2012 Feb241(2); 303-14.
- V. K. Krishnamurthy, A. M. Opoka, C. B. Kern, F. Guilak, D. A. Narmoneva, R. B. Hinton Maladaptive Matrix Remodeling and Regional Biomechanical Dysfunction in a Mouse Model of Aortic Valve Disease, Matrix Biology, 2012 Epup Jan12.
- L. Dupuis, D. R. McCulloch, J.D. McGarity, A. Bahn, A. Wessels, D. Weber, A. Megan Diminich, C.M. Nelson, S. S. Apte, C.B. Kern. Altered versican cleavage in ADAMTS5 deficient mice: A novel etiology of myxomatous valve disease. Developmental Biology 2011 Sep 1;357(1):152-64.
- R. Mukherjee, W.T. Rivers, J.M. Ruddy, R.G. Matthews, C.N. Koval, R.A. Plylar, E.I. Chang, R.K. Patal, C.B. Kern, R.E. Stroud, and F.G. Spinale. Long-Term Localized High-Frequency Electric Stimulation Within the Myocardial Infarct; Effects on Matrix Metalloproteinases and Regional Remodeling, Circulation 2010 Jul 6;122(1):20-32.
- C.B. Kern, A. Wessels, J. McGarity, L. Dixon, E. Alston, W.S. Argraves, D. Geeting, C.M. Nelson, D.R. Menick and S.S. Apte. Reduced versican cleavage due to Adamts9 haploinsufficiency is associated with cardiac and aortic anomalies, Matrix Biology, 2010 May; 29(4): 304-16. PMC2862783.
- S.K. Mani, E.A. Egan, B.K. Addy, M. Grimm, H. Kasiganesan, T. Thiyagarajan, L. Renaud, J.H. Brown, C.B. Kern and D. R. Menick. Beta-Adrenergic receptor stimulated Ncx1 upregulation is mediated via CaMKII/AP-1 signaling pathway in adult cardiomyocytes, J Mol Cell Cardiol, 2010 Feb;48(2):342-51. NIHMS165035.
- R. H. Anderson, R.P. Thompson and C. B. Kern. Development of aortic valves with 2 and 3 leaflets, Journal of the American College of Cardiology, 2009, Dec 8;54(24):2319-20.
- S. Chandrasekaran,# R. E. Peterson,# S. K. Mani, L. Renaud, A. L. Buchholz, B. Addy, L. Xu, T. Thiyagarajan, C. B. Kern and Donald R. Menick. Histone Deacetylases Facilitate Ncx1 Upregulation in Adult Cardiocytes 2009 Faseb Journal, Nov;23(11):3851-64
- B. Snarr, C.B. Kern, and A. Wessels. Origin and Fate of Cardiac Mesenchyme, Developmental Dynamics 2008 Oct; 237 Jul (10): 2804-19.
- M. A. Cooley, C.B. Kern, V.M. Fresco, A. Wessels, R.P. Thompson, T. C. McQuinn, W. O. Twal, C. H. Mjaatvedt, C. J. Drake and W. S. Argraves. Fibulin-1 is required for morphogenesis of neural crest-derived structures, Developmental Biology 2008 15;319 (2):336-45.
- E.W Wirrig, B.S. Snarr, A. L. Phelps, J. L. Barth, V. M. Fresco C.B. Kern, C.H. Mjaatvedt, B. P. Toole, S. Hoffman, T.C. Trusk, W.S. Argraves, and A. Wessels. Cartilage Link Protein 1 (Crtl1), an extracellular matrix component playing an important role in heart developmental Developmental Biology 2007 Oct. 15;310(2):291-303.
- C.B. Kern, R.A. Norris, R.P. Thompson, W.S. Argraves, S.E. Fairey, L. Reyes, S. Hoffman, R.R. Markwald and C.H. Mjaatvedt Versican proteolysis mediates myocardial regression during outflow tract development. Developmental Dynamics 2007 Jan16;236(3):671-683.
- C.B. Kern, W.O. Twal, C.H. Mjaatvedt, S.E. Fairey, B.P. Toole, M.L. Iruela-Arispe and W.S. Argraves. Proteolytic Cleavage of Versican During Cardiac Cushion Morphogenesis. Developmental Dynamics 2006 Aug;235(8):2238-47.
- C.H. Mjaatvedt, C.B. Kern, R.A. Norris, S. Fairey, and C.L. Cave. The Normal Distribution of Melanocytes in the Mouse Heart. Anatomical Record. Part A, Discoveries in Molecular, Cellular, & Evolutionary Biology 2005 285(A): 748-757.
- C.B. Kern, S. Hoffman, R. Moreno, B.J. Damon, R.A. Norris, E.L. Krug, R.R. Markwarld and C.H. Mjaatvedt. Immunolocalization of Chick Periostin Protein in the Developing Heart. Anatomical Record. Part A, Discoveries in Molecular, Cellular, & Evolutionary Biology. 2005 284A: 415-423.