Suniti Misra

Suniti Misra, Ph.D.
Assistant Professor

Member of Hollings Cancer Center

Room 613, Basic Science Building
Office: (843) 792-8642
Lab: 604 Children's Research Institute, (843) 876-5919

Email: misra@musc.edu
Misra CV


MS Univ. Massachusetts, 2004 Computer Science
PhD Calcutta Univ. (India), 1982 Biochemistry

Research Interests:

Fig1In general, inflammatory cancer cells are supported by a special type of connective tissue stroma that contains microvasculature networks that provide constant supplies of nutrients to inflammatory malignant cells. Hyaluronan (HA), a glycosaminoglycan, is one of the crucial components of such supportive tissue stroma. CD44, the major HA receptor, is encoded by single gene, but makes abundant use of alternative splicing to create functionally different CD44 variant isoforms, specifically, CD44v6, abbreviated here as HyaR. The standard isoform of CD44 (CD44s; without alternately spliced exons) is expressed predominantly in hematopoietic cells and normal epithelial cell subsets. Particularly, HyaR has been shown to contribute to tumor growth and metastatic processes, regulating oxidative stress in tissue fibrosis, and demonstrates cross-talk between matricellular protein and integrin signaling (see Fig.1).

fig 2We have recently invented a unique approach to deliver HyaR-shRNA tissue specifically to inhibit the tumor growth in colon. Short time delivery of HyaRshRNA reduces significantly intestinal/colon tumor number and growth in APC Min/+ mice bearing intestinal and colon tumors (Misra et.al. J. Biol. Chem. 2009) . Figure 2 shows steps involved in HyaRshRNA delivery in tumor cells using plasmids in transferrin coated nanoparticles. We used a combination of two plasmids – (i) intestine/colon specific plasmid pFabpl-Cre and (ii) pSico-HyaRshRNA. This combination reduced tumor sizes/ numbers in APC Min/+ mouse model (Misra et. al., 2009 J. Biol. Chem.). HyaRshRNA inhibits HyaR in the post-transcriptional step without altering CD44s. This approach of suppressing HyaR in colon tumors has deleterious effect on its downstream signaling as well as on three major receptor tyrosine kinases kinases c-Met, VEGFR, and EGFR that are the co-receptors of HyaR. This will be useful in future clinical application.

Outline of Research:

  • Molecular mechanisms of the regulation of chemo resistance in colon cancer by Hyaluronan receptor CD44v6 (HyaR) signaling.
  • Mechanisms of HyaR regulation of lipid-raft residing heteromeric-signaling complex
  • Regulation of receptor tyrosine kinases (RTKs that act as co-receptor to HyaR) downstream ant-apoptosis signaling by HyaR-signaling complex.
  • Mechanisms of HyaR-regulated TGFβ1-induced NADPH Oxidase/reactive oxygen species (ROS) in skin sclerosis and lung fibrosis.
  • Mechanisms of matricellular protein (Periostin and CCN1)-mediated mitral valve development pathways by Hyaluronan-CD44 signaling.


  1. Misra S, Hascall VC, Markwald RR, Ghatak S. Front Immunol. 2015 May 6;6:201. Review.  PMID: 25999946
  2. Ghatak S, Maytin EV, Mack JA, Hascall VC, Atanelishvili I, Moreno Rodriguez R, Markwald RR, Misra S. Roles of Proteoglycans and glycosaminoglycans in wound healing and fibrosis. Int J Cell Biol 2015, URL: http://www.hindawi.com/journals/ijcb/aa/834893/
  3. Misra S, Hascall VC, Markwald RR, Atanelishvili, Moreno-Rodriguez, Ghatak S. Utilization of GAGs/PGs as carriers for targeted therapy. Int J Cell Biol 2015, URL: http://www.hindawi.com/journals/ijcb/aa/537560/
  4. Misra S, Ghatak S, Vyas A, O'Brien P, Markwald RR, Khetmalas M, Hascall VC, McCarthy JB, Karamanos NK, Tammi MI, Tammi RH, Prestwitch GD, Padhye S.  Med Chem Res. 2014 Aug 1;23(8):3836-3851. PMID: 25013352
  5. Ghatak S, Misra S, Norris RA, Moreno-Rodriguez RA, Hoffman S, Levine RA, Hascall VC, Markwald RR. J Biol Chem. 2014 Mar 21;289(12):8545-61. PMID: 24469446
  6. Ghatak S, Bogatkevich GS, Atanelishvili I, Akter T, Feghali-Bostwick C, Hoffman S, Fresco VM, Fuchs JC, Visconti RP, Markwald RR, Padhye SB, Silver RM, Hascall VC, Misra S. J Biol Chem. 2014 Mar 14;289(11):7856-72. PMID:24324260
  7. Ghatak S, Vyas A, Misra S, O'Brien P, Zambre A, Fresco VM, Markwald RR, Swamy KV, Afrasiabi Z, Choudhury A, Khetmalas M, Padhye S. Bioorg Med Chem Lett. 2014 Jan 1;24(1):317-24. PMID: 24295787
  8. Kolpa HJ, Peal DS, Lynch SN, Giokas AC, Ghatak S, Misra S, Norris RA, Macrae CA, Markwald RR, Ellinor P, Bischoff J, Milan DJ. Development. 2013 May;140(10):2172-80. PMID: 23578931
  9. Misra S, Ghatak S, Patil N, Dandawate P, Ambike V, Adsule S, Unni D, Venkateswara Swamy K, Padhye S. Bioorg Med Chem. 2013 May 1;21(9):2551-9. PMID: 23517721
  10. Misra S, Heldin P, Hascall VC, Karamanos NK, Skandalis SS, Markwald RR, Ghatak S FEBS J. 2011 May; 278(9):1429-43. Review. PMID: 21362138
  11. Ghatak S, Hascall VC, Markwald RR, Misra S. J Biol Chem. 2010 Jun 25;285(26):19821-32. PMID: 20200161
  12. Misra S, Hascall VC, De Giovanni C, Markwald RR, Ghatak S. J Biol Chem. 2009 May 1; 284(18):12432-46. PMID: 19246453
  13. Toole BP, Ghatak S, Misra S. Curr Pharm Biotechnol. 2008 Aug;9(4):249-52. PMID: 18691085
  14. Ghatak S, Hascall VC, Berger FG, Penas MM, Davis C, Jabari E, He X, Norris JS, Dang Y, Markwald RR, Misra S. Connect Tissue Res. 2008;49(3):265-9. PMID: 18661357
  15. Misra S, Hascall VC, Berger FG, Markwald RR, Ghatak S. Connect Tissue Res. 2008;49(3):219-24. Review. PMID: 18661347
  16. Misra S, Obeid LM, Hannun YA, Minamisawa S, Berger FG, Markwald RR, Toole BP, Ghatak S. J Biol Chem. 2008 May 23;283(21):14335-44. PMID: 18326857
  17. Yokoyama U, Minamisawa S, Quan H, Ghatak S, Akaike T, Segi-Nishida E, Iwasaki S, Iwamoto M, Misra S, Tamura K, Hori H, Yokota S, Toole BP, Sugimoto Y, Ishikawa Y. J Clin Invest. 2006 Nov;116(11):3026-34. PMID: 17080198
  18. Misra S, Toole BP, Ghatak S. J Biol Chem. 2006 Nov 17;281(46):34936-41. PMID: 16959784
  19. Toole BP, Zoltan-Jones A, Misra S, Ghatak S. Cells Tissues Organs. 2005;179(1-2):66-72. Review. PMID: 15942194
  20. Misra S, Ghatak S, Toole BP. J Biol Chem. 2005 May 27;280(21):20310-5. PMID: 15784621
  21. Ghatak S, Misra S, Toole BP. J Biol Chem. 2005 Mar 11;280(10):8875-83. PMID: 15632176
  22. Marieb EA, Zoltan-Jones A, Li R, Misra S, Ghatak S, Cao J, Zucker S, Toole BP. Cancer Res. 2004 Feb 15;64(4):1229-32. PMID: 14983875
  23. Misra S, Ghatak S, Toole BP. J Biol Chem. 2005 May 27;280(21):20310-5. PMID: 15784621
  24. Ghatak S, Misra S, Toole BP. J Biol Chem. 2002 Oct 11;277(41):38013-20. PMID: 12145277
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Last updated on 29-Oct-2015

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